![]() These kits, mixture solutions, and collagen matrices fulfill a myriad of essential. Gel: Invitrogen Novex 420 Tris-Glycine mini gel, WedgeWell format left: prestained ladder after gel separation center: chemiluminescent substrate on western blot right: near-IR fluorescence on western blot. ![]() Use for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. Recommended ladders iBright Prestained Protein Ladder Invitrogen iBright Prestained Protein Ladder. Includes 6 protein bands from 8 to 160 kDa (see figure). Caution: Not intended for human or animal diagnostic or therapeutic uses.ġ- One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa.Ģ- Higher Added Value: Tri-colors and 10 bands with premium quality.ģ- Better performance for higher molecular weight proteins.Ĥ- Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system.ĥ- Enhanced transfer, wash, and stripping durabilities.Ħ2.5 mM Tris-H3PO4 (pH 7.5 at 25 ☌), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.ĥ μl of BLUltra Prestained Protein Ladder (PM001-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to the nitrocellulose membrane. Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.Proteins are used in routine laboratory procedures such as binding enzymes or coupling peptides to carrier proteins. The Chameleon 700 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 700 channel near-infrared detection. * supplement data should be considered for more accurate adjustment. Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Coloured reference bands serve as visual indicators of electrophoresis run progression and the efficiency of western transfer onto membranes following SDS-PAGE.
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